PubAg

Main content area

Unexpectedly fast transfer of positron-emittable artificial substrate into N-terminus of peptide/protein mediated by wild-type L/F-tRNA-protein transferase

Author:
Taki, Masumi, Kuroiwa, Hiroyuki
Source:
Amino acids 2015 v.47 no.6 pp. 1279-1282
ISSN:
0939-4451
Subject:
aminoacyl tRNA ligases, peptides, phenylalanine, positron-emission tomography, proteins
Abstract:
This article demonstrates the fastest enzymatic introduction of a positron emission tomography (PET) probe into acceptor peptides/proteins. It is site-specifically introduced at the basic N-terminus of the acceptors by using L/F-transferase in combination with aminoacyl-tRNA synthetase, namely the NEXT-A/PET reaction. Estimated from kinetic analysis, the transfer efficiency of O-(2-fluoromethyl)-L-tyrosine as an artificial amino acid PET probe mediated by the wild-type transferase is almost as good as that of the natural substrate, phenylalanine.
Agid:
1330386