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Development of a Loop‐Mediated Isothermal Amplification Assay to Detect Fusarium oxysporum

Author:
Lu, Chenchen, Dai, Tingting, Zhang, HaiFeng, Wang, YuanChao, Zheng, XiaoBo
Source:
Phytopathologische Zeitschrift 2015 v.163 no.1 pp. 63-66
ISSN:
0931-1785
Subject:
Fusarium oxysporum, Fusarium wilt, conidia, detection limit, fungi, genes, loop-mediated isothermal amplification, soil, soybeans, tissues
Abstract:
We report the development of a loop‐mediated isothermal amplification (LAMP) assay targeting the CYP51C element for visual detection of F. oxysporum which caused Fusarium wilt in soybean. The CYP51C‐LAMP assay efficiently amplified the target gene in 60 min at 62°C. And specificity was evaluated against F. oxysporum, Fusarium spp. and other fungal species. The detection limit of the CYP51C‐specific LAMP assay for F. oxysporum was four conidia per gram soil. The assay also detected F. oxysporum from inoculated soybean tissues and residues. These results suggest that this CYP51C‐LAMP assay can be used to detect residues on plants in the field.
Agid:
1331659