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Zinc glycine chelate absorption characteristics in Sprague Dawley rat

Yue, M., Fang, S. L., Zhuo, Z., Li, D. D., Feng, J.
Journal of animal physiology and animal nutrition 2015 v.99 no.3 pp. 457-464
absorption, alkaline phosphatase, blood serum, body weight, copper, gene expression, liver, males, messenger RNA, rats, small intestine, superoxide dismutase, tissues, transporters, tube feeding, zinc, zinc sulfate
This study was conducted to investigate absorption characteristics of zinc glycine chelate (Zn–Gly) by evaluating tissues zinc status and the expression of zinc transporters in rats. A total of 24 male rats were randomly allocated to three treatments and administered either saline or 35 mg Zn/kg body weight from zinc sulphate (ZnSO₄) or Zn–Gly by feeding tube separately. Four rats per group were slaughtered and tissues were collected at 2 and 6 h after gavage respectively. Our data showed that Zn–Gly did more effectively in increasing (p < 0.05) serum zinc levels, and the activities of serum and liver alkaline phosphatase (ALP) and liver Cu/Zn superoxide dismutase (Cu/Zn SOD) at 2 and 6 h. By 2 h after the zinc load, the mRNA and protein abundance of intestinal metallothionein1 (MT1) and zinc transporter SLC30A1 (ZnT1) were higher (p < 0.05), and zinc transporter SLC39A4 (Zip4) lower (p < 0.05) in ZnSO₄compared to other groups. Zinc transporter SLC39A5 (Zip5) mRNA expression was not zinc responsive, but Zip5 protein abundance was remarkably (p < 0.05) increased in ZnSO₄2 h later. Overall, our results indicated that in short‐term periods, Zn–Gly was more effective in improving body zinc status than ZnSO₄, and ZnSO₄did more efficiently on the regulation of zinc transporters in small intestine.