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Zinc glycine chelate absorption characteristics in Sprague Dawley rat

Author:
Yue, M., Fang, S. L., Zhuo, Z., Li, D. D., Feng, J.
Source:
Journal of animal physiology and animal nutrition 2015 v.99 no.3 pp. 457-464
ISSN:
0931-2439
Subject:
absorption, alkaline phosphatase, blood serum, body weight, copper, gene expression, liver, males, messenger RNA, rats, small intestine, superoxide dismutase, tissues, transporters, tube feeding, zinc, zinc sulfate
Abstract:
This study was conducted to investigate absorption characteristics of zinc glycine chelate (Zn–Gly) by evaluating tissues zinc status and the expression of zinc transporters in rats. A total of 24 male rats were randomly allocated to three treatments and administered either saline or 35 mg Zn/kg body weight from zinc sulphate (ZnSO₄) or Zn–Gly by feeding tube separately. Four rats per group were slaughtered and tissues were collected at 2 and 6 h after gavage respectively. Our data showed that Zn–Gly did more effectively in increasing (p < 0.05) serum zinc levels, and the activities of serum and liver alkaline phosphatase (ALP) and liver Cu/Zn superoxide dismutase (Cu/Zn SOD) at 2 and 6 h. By 2 h after the zinc load, the mRNA and protein abundance of intestinal metallothionein1 (MT1) and zinc transporter SLC30A1 (ZnT1) were higher (p < 0.05), and zinc transporter SLC39A4 (Zip4) lower (p < 0.05) in ZnSO₄compared to other groups. Zinc transporter SLC39A5 (Zip5) mRNA expression was not zinc responsive, but Zip5 protein abundance was remarkably (p < 0.05) increased in ZnSO₄2 h later. Overall, our results indicated that in short‐term periods, Zn–Gly was more effective in improving body zinc status than ZnSO₄, and ZnSO₄did more efficiently on the regulation of zinc transporters in small intestine.
Agid:
1331846