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Nocardiopsis mangrovei sp. nov., isolated from mangrove sediment

Huang, Hui-qin, Xing, Shan-shan, Yuan, Wei-dao, Wang, Ying, Liu, Min, Sun, Qian-guang, Lin, Xiang-zhi, Bao, Shi-xiang
Antonie van Leeuwenhoek 2015 v.107 no.6 pp. 1541-1546
DNA, Nocardiopsis trehalosi, bacteria, nucleotide sequences, pH, phylogeny, ribosomal RNA, sediments, sodium chloride, China
Two Gram-positive actinobacterial strains, designated HA11166ᵀand HA12420, were isolated from mangrove sediments in Hainan, China. The bacterial cells grew with 0–9 % (w/v) NaCl, at 15–40 °C and pH 5.0–10.0, with the optimum growth at 1 % NaCl, 30–37 °C and pH 7.0. The organisms had a range of chemical and morphological properties consistent with their classification in the genus Nocardiopsis. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strains HA11166ᵀand HA12420 can be affiliated to the genus Nocardiopsis and most closely related to Nocardiopsis trehalosi VKM Ac-942ᵀ(with the similarity of 97.2 and 97.5 %, respectively). The value of DNA–DNA relatedness between type strain HA11166ᵀ, selected as the representative strain, and N. trehalosi VKM Ac-942ᵀwas 38.8 %. The DNA G+C content of strain HA11166ᵀwas 73.7 %. On the basis of these phenotypic and genotypic data, strains HA11166ᵀand HA12420 are proposed to represent a novel species of the genus Nocardiopsis, for which the name Nocardiopsis mangrovei sp. nov. is proposed. The type strain is HA11166ᵀ(=CGMCC 4.7119ᵀ=DSM 46665ᵀ).