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Effect of Female Parent, Pollen Development, and Pollen Germinability on Absence of Seed Set in Avena sativa♀ ✕ 6x-Amphiploid♂; Pollinations

Forsberg, R. A., Nishiyama, I., Wang, S.
Crop science 1969 v.9 no.4 pp. 498-501
botany, Plant Science and Plant Products
Plants in A₇ – A₁₁ lines of a 6x amphiploid of Avena abyssinica Hochst. ✕ A. strigosa Schreb. were used as pollen parents in two crossing experiments. Females were A. sativa L. cultivars or three unique amphiploid-A. sativa backcross derivatives (D1, D2, D3). D1 had A. sativa cytoplasm while D2 and D3 had amphiploid cytoplasm. All crossing attempts were unsuccessful regardless of the female parent. This result led to an investigation of the development of pollen grains in the amphiploids and their germinability on A. sativa stigmas. The percentages of pollen grains filled with cytoplasm, among 18 panicles from 12 plants, ranged from 69.8 to 97.9. The average was 89.3 compared with 98.7% in A. sativa. The correlation between filled pollen grains and self-fertility in the same panicle was not significant (r = .11). Only 31.0% of the grains had three normal nuclei (Class I) compared with 81.2% in A. sativa. The correlation between percentage of Class I pollen and self-fertility in the same panicle was highly significant (r = .63). Most amphiploid pollen failed to germinate on A. sativa stigmas.