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Prposed Schemes for Mass-Extraction of Doubled Haploids of Cotton

Stelly, David M., Lee, Joshua A., Rooney, William L.
Crop science 1988 v.28 no.6 pp. 885-890
plant breeding, hybrids, alleles, haploidy, Gossypium hirsutum, colchicine, interspecific hybridization
We propose (i) synthesis of hybrid-eliminating, haploid-inducing (HEHP) lines and populations homozygous for the Le₂ ᵈᵃʸ hybrid lethality allele and the Se allele for semigamous reproduction, (ii) cross pollination of HEHP plants with pollen of normal cotton (Gossypium hirsutum and G. barbadense) to form inviable true hybrids, but viable haploids, and (iii) bulk treatment of all seed or young viable seedlings with colchicine to recover doubled haploids en masse. Doubled paternal haploid plants and sectors will be phenotypically unique, as distinguished by fertility, size of plant parts, and expression of genetic marker(s). Inclusion of male sterility in segregating HEHP populations will facilitate manual or natural cross pollination, allowing large-scale usage. To convert cytoplasms of HEHP materials, available semigamous lines with the desired cytoplasm can be crossed with a HEHP pollen parent to obtain paternal haploid progeny that can be colchicine-doubled. The HEHP materials should facilitate all types of cytogenetic manipulations involving extraction of doubled (paternal) haploids. The basic concepts and methods proposed might be extrapolatable to other crops. Joint contribution of the Texas Agric. Exp. Stn., the North Carolina Agric. Res. Serv., and the USDA-ARS. Texas Agric. Exp. Stn. manuscript TA-21500. Supported in part by USDA CRGO grants 84-CRCR-l-1516 and 86-CRCR-1-2129.