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Use of Radiation to Transfer Alien Chromosome Segments to Wheat

Author:
Sears, E. R., Gustafson, J. P.
Source:
Crop science 1993 v.33 no.5 pp. 897-901
ISSN:
0011-183X
Subject:
Triticum aestivum, cytogenetics, chromosomes, genes, chromosome translocation, gene transfer, chromosome substitution, chromosome pairing, ionizing radiation
Abstract:
Ionizing radiation can accomplish the transfer of genetic information from species so distantly related to wheat (L. em Thell.) that their chromosomes pair very little, if at all, with those of wheat, even in the absence of the homoeologous-pairing suppressor 1. In a successful transfer, the alien segment must almost always replace a homoeologous wheat segment, but radiation induces translocations largely at random; therefore automatic selection in favor of desirable translocations must be provided if the size of the project is to be kept within reasonable limits. Pollen selection will occur if seeds or plants monosomic for both an alien chromosome and one of its wheat homoeologues are irradiated. Making the plants also deficient for 1 may increase the number of suitable transfers. High-frequency occurrence of the desired alien character in M head-rows from plants grown from irradiated seed can identify favorable transfers with little cytological work. Irradiation of plants shortly before meiosis, using them to pollinate ditelosomics or double ditelosomics for the wheat arm or chromosome concerned, and cytologically examining offspring which have the alien character can not only identify the desirable transfers, but also reveal the lengths of the alien segments involved.
Agid:
1339260