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The Preparation and Ethanol Fermentation of High-Concentration Sugars from Steam-Explosion Corn Stover

Author:
Xie, Hui, Wang, Fengqin, Yin, Shuangyao, Ren, Tianbao, Song, Andong
Source:
Applied biochemistry and biotechnology 2015 v.176 no.2 pp. 613-624
ISSN:
0273-2289
Subject:
Pachysolen tannophilus, Saccharomyces cerevisiae, bioethanol, carbon, cellulosic materials, corn stover, ethanol, ethanol fermentation, hydrolysis, reducing sugars, saccharification, yeasts
Abstract:
In the field of biofuel ethanol, high-concentration- reducing sugars made from cellulosic materials lay the foundation for high-concentration ethanol fermentation. In this study, corn stover was pre-treated in a process combining chemical methods and steam explosion; the cellulosic hydrolyzed sugars obtained by fed-batch saccharification were then used as the carbon source for high-concentration ethanol fermentation. Saccharomyces cerevisiae 1308, Angel yeast, and Issatchenkia orientalis were shake-cultured with Pachysolen tannophilus P-01 for fermentation. Results implied that the ethanol yields from the three types of mixed strains were 4.85 g/100 mL, 4.57 g/100 mL, and 5.02 g/100 mL (separately) at yield rates of 91.6, 89.3, and 92.2 %, respectively. Therefore, it was inferred that shock-fermentation using mixed strains achieved a higher ethanol yield at a greater rate in a shorter fermentation period. This study provided a theoretical basis and technical guidance for the fermentation of industrial high-concentrated cellulosic ethanol.
Agid:
1347545