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Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats
- Suzuki, Takehito, Fukuoka, Hideo, Ushikoshi, Setsuo, Sato, Reiichiro, Morita, Hidetoshi, Takizawa, Tatsuya
- Animal science journal = 2015 v.86 no.5 pp. 532-540
- Rhizopus oryzae, alanine transaminase, aspartate transaminase, blood serum, body weight, carbon tetrachloride, gene expression, hepatocyte growth factor, hepatocytes, hepatoprotective effect, insulin-like growth factor I, lactate dehydrogenase, liver, oral administration, rats, reverse transcriptase polymerase chain reaction
- Hepatoprotective effects of Rhizopus oryzae/ U‐1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl₄)‐induced liver‐injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100 mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl₄(125 mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl₄. (ii) RU pretreatment (100 mg/kg) increased 5‐bromo‐2′‐deoxyuridine incorporation at 48 h after CCl₄treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl₄administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin‐like growth factor‐I (IGF‐I) gene expression in hepatocytes injured by CCl₄treatment. From these results, we conclude that oral pre‐administration of RU was effective to suppress liver injury induced by the subsequent oral CCl₄administration, and RU‐induced increase in IGF‐I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.