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Protective effect of aqueous extracts from Rhizopus oryzae on liver injury induced by carbon tetrachloride in rats

Suzuki, Takehito, Fukuoka, Hideo, Ushikoshi, Setsuo, Sato, Reiichiro, Morita, Hidetoshi, Takizawa, Tatsuya
Animal science journal = 2015 v.86 no.5 pp. 532-540
Rhizopus oryzae, alanine transaminase, aspartate transaminase, blood serum, body weight, carbon tetrachloride, gene expression, hepatocyte growth factor, hepatocytes, hepatoprotective effect, insulin-like growth factor I, lactate dehydrogenase, liver, oral administration, rats, reverse transcriptase polymerase chain reaction
Hepatoprotective effects of Rhizopus oryzae/ U‐1 aqueous extract (RU) were demonstrated in carbon tetrachloride (CCl₄)‐induced liver‐injured rats. In order to investigate the RU effects, the rats were administered RU at a dose of 10 or 100 mg/kg of body weight for 10 days before induction of the liver injury by oral administration of CCl₄(125 mg/kg body weight). (i) Pretreatment with RU caused a significant decrease in serum lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities that were increased by the administration of CCl₄. (ii) RU pretreatment (100 mg/kg) increased 5‐bromo‐2′‐deoxyuridine incorporation at 48 h after CCl₄treatment in hepatocytes. (iii) Histological hematoxylin and eosin staining of the liver showed that RU pretreatment reduced the damage induced by CCl₄administration. (iv) Reverse transcriptase PCR analysis showed RU retreatment caused a transient but significant increase in hepatocyte growth factor (HGF) and a sustained and significant increase in insulin‐like growth factor‐I (IGF‐I) gene expression in hepatocytes injured by CCl₄treatment. From these results, we conclude that oral pre‐administration of RU was effective to suppress liver injury induced by the subsequent oral CCl₄administration, and RU‐induced increase in IGF‐I and HGF gene expression may be, even in part, involved in biological actions of RU in rats.