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Bacterial community of pond's water, sediment and in the guts of tilapia (Oreochromis niloticus) juveniles characterized by fluorescent in situ hybridization technique
- Del'Duca, Alessandro, Cesar, Dionéia Evangelista, Abreu, Paulo César
- Aquaculture research 2015 v.46 no.3 pp. 707-715
- Lactobacillus brevis, Lactobacillus collinoides, Lactobacillus coryniformis, Lactobacillus farciminis, Oreochromis niloticus, Pseudomonas fluorescens, Vibrio, aquaculture systems, bacteria, bacterial communities, community structure, digestive tract, fish, fish culture, fluorescence, fluorescence in situ hybridization, gastrointestinal system, juveniles, microbial detection, monitoring, ponds, probiotics, sediments
- Information about bacterial community structure and functioning in fish farming ponds remains scarce, mainly due to methodological difficulties in counting and identifying uncultured bacteria. The main objective of this study was to evaluate the degree of similarity between the bacterial community of the digestive tract of tilapia (Oreochromis niloticus) juveniles and that of the test pond's water and sediment, using the Fluorescent In Situ Hybridization (FISH) technique. Samples of water, sediment and gut content of 30 tilapia juveniles from a single nursery ground were collected in January 2010. Potentially probiotic and pathogenic bacteria of the species Bacillus, Lactobacillus brevis, Lactobacillus collinoides, Lactobacillus coryniformis, Lactobacillus farciminis, Vibrio and Pseudomonas fluorescens were found in different samples using specific fluorescent probes. The similarity between bacterial community environments and gastrointestinal tracts was determined using the Morisita‐Horn index. The fish guts presented higher abundances of Pseudomonas fluorescens, Bacillus, Lactobacillus brevis and Lactobacillus collinoides. The bacterial community composition of tilapia gastrointestinal tract was more similar to the water than the sediment of the pond. The results of this study showed that the FISH technique can be easily used for monitoring of probiotics and pathogen detection in aquaculture systems.