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Biochemical composition and fatty acid profile in a strain of the lineage ‘Nevada’, belonging to the Brachionus plicatilis (Rotifera) complex, fed different diets

Author:
Kostopoulou, Venetia, Miliou, Helen, Verriopoulos, George
Source:
Aquaculture research 2015 v.46 no.4 pp. 813-824
ISSN:
1355-557X
Subject:
Brachionus plicatilis, DNA, RNA, bakers yeast, diet, diurnal variation, docosahexaenoic acid, eicosapentaenoic acid, embryogenesis, fatty acid composition, highly unsaturated fatty acids, lipid content, monounsaturated fatty acids, omega-3 fatty acids, parthenogenesis, population structure, protein content, reproductive performance, Nevada
Abstract:
A strain of the lineage Brachionus ‘Nevada’ was batch cultured with two diets, differing in biochemical composition: baker's yeast (treatment 1), which has higher protein:lipid ratio compared with CULTURE SELCO (treatment 2). The biochemical composition (DNA, RNA, lipid, protein content) and fatty acid profile of rotifers of both treatments was analysed and related to previously published population structure data. CULTURE SELCO‐fed rotifers showed higher DNA, RNA, lipid, n‐3 highly unsaturated fatty acids (HUFA) average content, compared with yeast‐fed rotifers, which had higher protein content. Rotifer lipid content showed significant diurnal variation in yeast‐fed rotifers. Rotifer lipid and n‐3 HUFA content was associated with reproductive output. DNA and RNA content was related to embryonic development while protein content, to somatic growth and mixis. The saturated and monounsaturated fatty acid rotifer content was stable irrespective of feed, in contrast to eicosapentanoic acid (EPA) and DHA. The levels of AA were similar in both rotifer populations, but those of EPA and docosahexaenoic acid (DHA) were about half in yeast‐fed compared with CULTURE SELCO‐fed rotifers. CULTURE SELCO resulted in a temporally stable rotifer lipid profile and a better enriched parthenogenetic population.
Agid:
1358807