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Facile spectrophotometric quantification method of sinapic acid in hexane-extracted and methanol-ammonia-water-treated mustard and rapeseed meals

Author:
Naczk, M., Wanasundara, P.K.J.P.D., Shahidi, F.
Source:
Journal of agricultural and food chemistry 1992 v.40 no.3 pp. 444-448
ISSN:
0021-8561
Subject:
spectroscopy, Brassica juncea, Brassica napus, cultivars, mustard meal, rapeseed meal, extraction, hexane, phenolic acids, sinapic acid
Abstract:
The contribution of sinapic acid to the free, esterified, and insoluble bound phenolics of a number of Brassica oilseeds, namely Triton (B. napus), Midas (B. napus), and Hu You 9 (B. napus) rapeseed and Domo mustard (B. juncea), was determined according to a simple spectrophotometric method. Sinapic acid constituted 65-86% of free and 71-97% of esterified phenolic acids of defatted meals. The contribution of sinapic acid to insoluble bound phenolic was 7-32%. Treatment of defatted meals with 10% ammonia in 95% methanol (MeOH-NH3-H2O) resulted in the removal of 41-75% of their total free phenolics and 45-79% of the content of sinapic acid in this fraction. This process also removed 82-93% of the total esterified phenolics and 86-93% of their corresponding sinapic acid. In all cases, MeOH-NH3-H2O extraction enhanced the protein content of the resultant meals.
Agid:
1360142