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Isolation and characterization of pseudobactin B: a pseudobactin-type siderophore from Pseudomonas species strain PD 30
- Andriollo, N., Guarini, A., Cassani, G.
- Journal of agricultural and food chemistry 1992 v.40 no.7 pp. 1245-1248
- purification, Pseudomonas, cell culture, fermentation, fractionation, spectral analysis, siderophores
- A simple method is discussed for the isolation of pseudobactin B, a new siderophore produced together with pseudobactin by plant growth promoting bacterium Pseudomonas PD 30. Pseudobactin B differs from the known pseudobactin only by the nature of the dicarboxylic acid attached to the chromophore, which is succinic acid instead of succinamide. The structure of pseudobactin B was determined by a comparison of its 1H NMR, UV, and mass spectra with those of pseudobactin. An HPLC assay for pseudobactin B and pseudobactin, quantification in fermentation beer, and a new efficient method for isolating them are proposed.