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Advanced technique for three-dimensional visualization of compound distributions in a rice kernel

Author:
Ogawa, Y., Sugiyama, J., Kuensting, H., Ohtani, T., Hagiwara, S., Liu, X.Q., Kokubo, M., Yamamoto, A., Kudoh, K., Higuchi, T.
Source:
Journal of agricultural and food chemistry 2001 v.49 no.2 pp. 736-740
ISSN:
0021-8561
Subject:
rice, image analysis, histochemistry, starch, lipids, plant proteins, spatial distribution
Abstract:
A three-dimensional (3D) visualization technique for the compound distribution in a rice kernel was developed. This technique is a combination of sectioning, staining, and digital image postprocessing. By using a special microtome system with adhesive tapes, a set of sequential sections of a rice kernel, which can be preserved with their own set of relative position data, was obtained. A single set of sequential sections was stained by various chemical techniques for the visualization of protein, starch, or lipid content. Each stained section was digitally captured using a CCD imaging device. As the stained areas represent areas containing dye-target complex, the distribution of each compound in the section was visualized in two dimensions. The digitally captured images of a single set of sequential sections were reconstructed to produce a 3D plotting image. As a result, the distributions of various compounds in a rice kernel could be visualized in a new 3D model.
Agid:
1362008