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Capillary zone electrophoresis of coniferyl alcohol oxidation products
- Fournand, D., Lapierre, C.
- Journal of agricultural and food chemistry 2001 v.49 no.12 pp. 5727-5731
- phenolic compounds, alcohols, electrophoresis, oxidation, enzyme activity, peroxidase, pH, temperature
- Capillary zone electrophoresis (CZE) was developed for the quantitative determination of dimers obtained by horseradish peroxidase-catalyzed oxidation of coniferyl alcohol. The influence of pH, electrolyte concentration, applied voltage, and temperature on CZE performance was investigated, resulting in an efficient and rapid separation. Coniferyl alcohol-derived dimers were directly analyzed from their reaction mixtures, without any extraction or derivatization step. In addition, these dimers were analyzed within 14 min, a substantially shorter time than is required for the HPLC method or the conventional capillary gas chromatography of their silylated derivatives. Standard deviations between injection replicates were in the 0.4-0.7% range for migration times and in the 1.8-5.1% range for relative normalized peak areas. The method could therefore be successfully applied to follow the peroxidase-catalyzed oxidation of coniferyl alcohol.