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Highly polymerized procyanidins in brown soybean seed coat with a high radical-scavenging activity

Takahata, Y., Ohnishi-Kameyama, M., Takahashi, M., Suda, I.
Journal of agricultural and food chemistry 2001 v.49 no.12 pp. 5843-5847
testa, proanthocyanidins, free radicals, polymerization, soybeans, varieties, color, polymers, anthocyanidins
1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity of the 70% aqueous acetone extract from the seed coat of the brown soybean variety, Akita-Zairai, was investigated. The activity of the seed coat of Akita-Zairai was much higher than that of three other reddish-brown varieties, but lower than that of two black varieties, and was closely dependent on the content of phenolic compounds. In the LH20 column chromatography of Akita-Zairai, high DPPH radical-scavenging activities were detected in the fractions eluted with MeOH and 70% aqueous acetone. Proanthocyanidins were also detected in fractions showing high radical-scavenging activities. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry analysis showed that the degree of polymerization (DP) of the procyanidins contained in the brown or black soybean seed coat was as high as DP30.