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Isolation and determination of anthocyanins in seed coats of black soybean (Glycine max (L.) Merr.)
- Choung, M.G., Baek, I.Y., Kang, S.T., Han, W.Y., Shin, D.C., Moon, H.P., Kang, K.H.
- Journal of agricultural and food chemistry 2001 v.49 no.12 pp. 5848-5851
- soybeans, color, anthocyanins, glucosides, chemical composition, chemical structure, varieties, testa
- Anthocyanin pigments in seed coats of black soybean (Glycine max (L.) Merr.) were extracted with 1% HCl-CH3OH, and the crude anthocyanin extract was purified by Sephadex LH-20 and Lichroprep RP-18 open-column chromatography. Three major anthocyanins were isolated, and their chemical structures were identified by spectroscopic methods (UV-visible, FABMS, 1H and 13C NMR, and by TLC). The complete structures of these anthocyanins were elucidated as delphinidin-3-glucoside, cyanidin-3-glucoside, and petunidin-3-glucoside. Among them, petunidin-3-glucoside was identified as a new anthocyanin in black soybeans. On the basis of RP-HPLC with a UV-vis detector, the contents of delphinidin-3-glucoside, cyanidin-3-glucoside, petunidin-3-glucoside, and total anthocyanins in seed coats of 10 black soybeans were found in the ranges of 0-3.71, 0.94-15.98, 0-1.41, and 1.58-20.18 mg/g, respectively. The results obtained in this study imply that the seed coats of black soybean can be used as a good source for cyanidin-3-glucoside and delphinidin-3-glucoside.