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Correlations between pulp properties of Eucalyptus clones and leaf volatiles using automated solid-phase microextraction

Zini, C.A., Assis, T.F. de, Ledford, E.B. Jr., Dariva, C., Fachel, J., Christensen, E., Pawliszyn, J.
Journal of agricultural and food chemistry 2003 v.51 no.27 pp. 7848-7853
Eucalyptus, clones, hybrids, leaves, volatile organic compounds, essential oils, solid phase extraction, microextraction, automation, pulpwood, pulping, wood pulp, statistical analysis, principal component analysis, alpha-terpineol, sesquiterpenoids, gas chromatography, mass spectrometry, cluster analysis
Analysis of biogenic volatile organic compounds (BVOC) of 14 Eucalyptus clones has been performed using an automated headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography (GC)/ion trap mass spectrometry (ITMS) method. Correlations between pulp properties of Eucalyptus clones and the BVOC of their leaf headspaces were studied. The compounds α-terpineol and the sesquiterpene β-eudesmol were positively correlated with S5, a property related to the hemicelluose content in the pulp. Qualitative results obtained with automated HS-SPME were sufficient to group together the same species and related hybrids through cluster analysis and were confirmed through principal component analysis. A preliminary separation of the essential oils of Eucalyptus dunnii through comprehensive two-dimensional gas chromatography (GC x GC) showed ~580 peaks compared to ~60 in a typical GC/ITMS first-dimension chromatogram. The potential of HS-SPME coupled to GC x GC to improve the separation of Eucalyptus volatiles and other plant essential oils looks extremely promising for new applications of unsupervised learning methods.