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FliZ Acts as a Repressor of the ydiV Gene, Which Encodes an Anti-FlhD4C2 Factor of the Flagellar Regulon in Salmonella enterica Serovar Typhimurium

Wada, Takeo, Tanabe, Yasushi, Kutsukake, Kazuhiro
Journal of bacteriology 2011 v.193 no.19 pp. 5191-5198
Salmonella enterica subsp. enterica serovar Typhimurium, mutants, operon, promoter regions, regulon, transcription (genetics)
YdiV acts as an anti-FlhD4C2 factor, which negatively regulates the class 2 flagellar operons in poor medium in Salmonella enterica serovar Typhimurium. On the other hand, one of the class 2 flagellar genes, fliZ, encodes a positive regulator of the class 2 operons. In this study, we found that the FliZ-dependent activation of class 2 operon expression was more profound in poor medium than in rich medium and not observed in the ydiV mutant background. Transcription of the ydiV gene was shown to increase in the fliZ mutant. Purified FliZ protein was shown in vitro to bind to the promoter region of the nlpC gene, which is located just upstream of the ydiV gene, and to repress its transcription. These results indicate that FliZ is a repressor of the nlpC-ydiV operon and activates the class 2 operons by repressing ydiV expression. Therefore, the fliZ and ydiV genes form a regulatory loop.