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Coupling- and repulsion-phase RAPDs for marker-assisted selection of PI 181996 rust resistance in common bean

Johnson, E., Miklas, P.N., Stavely, J.R., Martinez-Cruzado, J.C.
Theoretical and applied genetics 1995 v.90 no.5 pp. 659
Phaseolus vulgaris, genetic markers, genes, disease resistance, Uromyces appendiculatus, rust diseases, lines, plant breeding, cultivars, introgression, linkage (genetics), artificial selection, random amplified polymorphic DNA technique
The Guatemalan black bean (Phaseolus vulgaris L.) plant introduction (PI) 181996 is resistant to all known US races of the bean rust fungus Uromyces appendiculatus (Pers. ex Pers.) Unger var. appendiculatus [syn. U. phaseoli (Reben) Wint.]. We report on two random amplified polymorphic DNA (RAPD) markers OAC20(490) tightly linked (no recombinants) in coupling phase and OAE19(890) linked in repulsion phase (at 6.2 +/- 2.8 cM) to PI 181996 rust resistance. These RAPDs, generated by single decamer primers in the polymerase chain reaction, were identified in near-isogenic bulks of non-segregating resistant and susceptible BC4F2 (NX-040*4/PI 181996) lines. Linkage of the RAPD markers was confirmed by screening 19 BC4F2 and 57 BC4F3 individuals segregating for PI 181996 resistance. Utility of the RAPDs OAC20(490) and OAE19(890) was investigated in a diverse group of common bean cultivars and lines. All cultivars into which the PI 181996 resistance was introgressed had the RAPD OAC20(490). A RAPD similar in size to OAC20(490), observed in some susceptible common bean lines, was confirmed by Southern blotting to be homologous to the RAPD OAC20(490). Use of the RAPDs OAC20(490) and OAE19(890) in marker-assisted selection (MAS) is proposed. The coupling-phase RAPD is most useful for MAS of resistant BCnF1 individuals during traditional backcross breeding. The repulsion-phase RAPD has greatest utility in MAS of homozygous-resistant individuals in F2 or later-segregating generations.