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Determination of oleandrin in tissues and biological fluids by liquid chromatography-electrospray tandem mass spectrometry

Tor, E.R., Filigenzi, M.S., Puschner, B.
Journal of agricultural and food chemistry 2005 v.53 no.11 pp. 4322-4325
Nerium oleander, cardiac glycosides, chemical residues, blood serum, urine, animal tissues, liquid chromatography, mass spectrometry, liver, poisonous plants, poisoning, disease diagnosis, cattle
A rapid LC-MS/MS method, using a triple-quadrupole/linear ion trap mass spectrometer, was developed for the quantitative determination of oleandrin in serum, urine, and tissue samples. Oleandrin, the major cardiac glycoside of oleander (Nerium oleander L.), was extracted from serum and urine samples with methylene chloride and from tissues with acetonitrile. The tissue extracts were cleaned up using Florisil solid-phase extraction columns. Six replicate fortifications of serum and urine at 0.001 microgram/g (1 ppb) oleandrin gave average recoveries of 97% with 5% CV (relative standard deviation) and 107% with 7% CV, respectively. Six replicate fortifications of liver at 0.005 microgram/g (5 ppb) oleandrin gave average recoveries of 98% with 6% CV. This is the first report of a positive mass spectrometric identification and quantitation of oleandrin in tissue samples from oleander intoxication cases. The sensitivity and specificity of the LC-MS/MS analysis enables it to be the method of choice for toxicological investigations of oleander poisoning.