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Isolation and identification of oligosaccharide in a commercial beet medium invert syrup

Author:
Swallow, K.W., Low, N.H.
Source:
Journal of agricultural and food chemistry 1993 v.41 no.10 pp. 1587-1592
ISSN:
0021-8561
Subject:
syrups, sugar beet, oligosaccharides, kestose, purification, nuclear magnetic resonance spectroscopy, sucrose
Abstract:
Research was conducted to isolate and identify oligosaccharides in a commercial beet medium invert syrup BMIS). Isolation of two BMIS oligosaccharides was accomplished using charcoal/Celite chromatography, preparative high-performance liquid chromatography, and gel permeation chromatography. Partial and total acid hydrolysis, spray reagents for reducing and ketose-containing carbohydrates, selective enzymatic hydrolysis, and 13C nuclear magnetic resonance spectroscopy were used to identify these oligosaccharides. 6-Kestose (O-beta-D-fructofuranosyl-(2 leads to 6)-beta-D-fructofuranosyl-alpha-D-glucopyranoside) and the previously unidentified carbohydrate, kelose (O-alpha-D-fructofuranosyl-(2 leads to 6)-beta-D-fructofuranosyl-alpha-D-glucopyranoside), were identified as two of the oligosaccharides in a commercial BMIS.
Agid:
1367852