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Endochitinases from Castanea crenata cotyledons
- Collada, C., Casado, R., Aragoncillo, C.
- Journal of agricultural and food chemistry 1993 v.41 no.10 pp. 1716-1718
- Castanea crenata, chitinase, isozymes, molecular weight, enzyme activity, cotyledons, amino acids, amino acid composition
- Three constitutive endochitinase isoforms were purified from the cotyledons of Castanea crenata. All three isoforms are basic with pI between 8.5 and 9, possess approximate molecular weights of 25 000, 26 000, and 25 500, and have been named Cha, Chb, and Chc, respectively. All isoforms cross-reacted with antibodies raised against Ch1 chitinase previously purified from Castanea sativa. The molecular weights and amino acid compositions of Cha, Chb, and Chc suggest that these proteins belong to the class II endochitinases.