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Heme protein oxidation and lipid peroxidation of tissue homogenates induced by halogenated hydrocarbon, hydroperoxide, and transition metals

Author:
Chen, H., Tappel, A.L.
Source:
Journal of agricultural and food chemistry 1993 v.41 no.9 pp. 1362-1367
ISSN:
0021-8561
Subject:
chlorinated hydrocarbons, liver, copper, organobromine compounds, heme, lipid peroxidation, spleen, oxidation, kidneys, rats, heart, metal ions
Abstract:
Damage of liver, heart, kidney, and spleen caused by spontaneous oxidative reaction or oxidative reactions induced by halogenated hydrocarbon, hydroperoxide, or transition metals was studied in a tissue homogenate system. Both thiobarbituric acid reactive substances (TBARS) and oxidized heme proteins (OHP) were measured in oxidatively damaged tissue homogenates. Bromotrichloromethane (CBrCl3), tert-butyl hydroperoxide (TBHP), ferrous iron (Fe2+), and cupric ion (Cu2+) are potent prooxidants. In general, the production of OHP increased more rapidly than the production of TBARS, especially with short incubation periods and low concentrations of prooxidants. The correlation between heme protein oxidation and lipid peroxidation and the merits of using OHP to measure oxidative damage of tissues are discussed.
Agid:
1368107