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Formation and inhibition of genotoxic glyoxal and malonaldehyde from phospholipids and fish liver oil upon lipid peroxidation

Nishiyama, T., Hagiwara, Y., Hagiwara, H., Shibamoto, T.
Journal of agricultural and food chemistry 1994 v.42 no.8 pp. 1728-1731
Hordeum vulgare, barley, leaves, flavonoids, malondialdehyde, aldehydes, cod liver oil, phospholipids, lipid peroxidation, antioxidants
Many aldehydes formed from lipid peroxidation are reportedly implicated in various diseases, including arteriosclerosis. Lecithin, a blood plasma lipid, and cod liver oil (known to have a beneficial effect on coronary artery disease) were oxidized by Fenton's reagent. The oxidation products, malonaldehyde (MA) and glyoxal, were analyzed in the presence of alpha-tocopherol or 2"-O-glycosylisovitexin (2"-O-GIV) by gas chromatography after derivatization to 1-methylpyrazol and quinoxaline, respectively. alpha-Tocopherol inhibited MA and glyoxal formation effectively from 5 mg of lecithin at low doses (1 micromole), whereas 2"-O-GIV required 6 micromole to produce the same level of inhibition. However, alpha-tocopherol did not exhibit a satisfactory effect in the case of cod liver oil compared with 2"-O-GIV. Moreover, addition of alpha-tocopherol to cod liver oil increased glyoxal formation slightly. The results of the present study suggested that 2"-O-GIV is an excellent antioxidant for fish oils.