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PsMPK7, a stress‐associated mitogen‐activated protein kinase (MAPK) in Phytophthora sojae, is required for stress tolerance, reactive oxygenated species detoxification, cyst germination, sexual reproduction and infection of soybean

Author:
Gao, Jian, Cao, Mingna, Ye, Wenwu, Li, Haiyang, Kong, Liang, Zheng, Xiaobo, Wang, Yuanchao
Source:
Molecular plant pathology 2015 v.16 no.1 pp. 61-70
ISSN:
1464-6722
Subject:
Phytophthora sojae, aniline, genes, germination, hydrogen peroxide, laccase, mitogen-activated protein kinase, oospores, oxidative stress, pathogenicity, phosphorylation, plant pathogenic fungi, secretion, sexual reproduction, signal transduction, sodium chloride, sorbitol, soybeans, stress tolerance, zoospores
Abstract:
The sensing of stress signals and their transduction into appropriate responses are crucial for the adaptation, survival and infection of phytopathogenic fungi and oomycetes. Amongst evolutionarily conserved pathways, mitogen‐activated protein kinase (MAPK) cascades function as key signal transducers that use phosphorylation to convey information. In this study, we identified a gene, designated PsMPK7, one of 14 predicted genes encoding MAPKs in Phytophthora sojae. PsMPK7 was highly transcribed in each tested stage, but was up‐regulated in the zoospore, cyst and cyst germination stages. Silencing of PsMPK7 affected the growth of germinated cysts, oospore production and the pathogenicity of soybean. PsMPK7 transcription was induced by stresses from sorbitol, NaCl and hydrogen peroxide. Transformants in which PsMPK7 expression was silenced (PsMPK7‐silenced) were significantly more sensitive to osmotic and oxidative stress. Aniline blue and diaminobenzidine staining revealed that the silenced lines did not suppress the host reactive oxygen species (ROS) burst, indicating that either the inoculated plants activated stronger defence responses to the transformants and/or the PsMPK7‐silenced transformants failed to overcome plant defences. In addition, extracellular secretion of laccase decreased in the silenced lines. Overall, our results indicate that the PsMPK7 gene encodes a stress‐associated MAPK in P. sojae that is important not only for responses to various stresses, but also for ROS detoxification, cyst germination, sexual oospore production and infection of soybean.
Agid:
1377842