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Reduction of adenosine triphosphate in eggs of Fuller rose beetle (Coleoptera: Curculionidae) induced by lethal temperature and methyl bromide

Forney, C.F., Aung, L.H., Brandl, D.G., Soderstrom, E.L., Moss, J.I.
Journal of economic entomology 1991 v.84 no.1 pp. 198-201
Citrus, Curculionidae, mortality, ova, viability, adenosine triphosphate, rapid methods, temperature, California
The concentration of adenosine triphosphate (ATP) in relation to the viability of Fuller rose beetle, Asynonychus godmani Crotch, eggs was determined with the firefly, Photinus pyralis (L.), bioluminescent assay. ATP concentrations decreased significantly when eggs were killed by freezing, hot water, or methyl bromide fumigation. Two hours after eggs were frozen in liquid nitrogen, the ATP concentration was only 5% that of unfrozen, live eggs. The ATP concentration decreased logarithmically for the first 30 min after freezing; the ATP half-life was estimated to be 12.9 min. ATP concentrations in eggs killed by a 3-min dip in 55 degrees C water dropped 40% 2 h after treatment. In eggs fumigated for 2 h with 80 g/m3 methyl bromide, the ATP concentration did not decrease for 8 h following treatment. However, after 24 h, ATP concentrations were 50% less than the nonfumigated eggs. The Fuller rose beetle eggs killed by all three treatments contained significantly less ATP than live eggs 24 h following treatment. Thus, the results suggest that the firefly bioluminescent ATP assay could serve as a rapid screening method for ascertaining Fuller rose beetle egg viability.