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A method for hyperthermic treatment of mouse skin
- Gragtmans, N. J., Jevcak, J. J., Mitchel, R. E. J., Morrison, D. P., McCann, R. A., Murphy, J. W.
- Laboratory animals 1992 v.26 no.2 pp. 122-126
- mice, skin, animal models, disease models, fever, carcinogenesis, carcinogens, promoter regions
- The Sencar mouse skin system is a recognized model for tumour initiation, promotion and progression. The current interest in the effect of hyperthermia on this multi-stage tumorigenesis model prompted the need for a technique to accurately heat a section of dorsal skin of a large number of mice for 30 min per heat treatment. In the technique described, experimental groups of 25 female Sencar mice were treated at 7-8 weeks of age under general methoxyflurane anaesthesia. Treatment consisted of the application of initiating and/or promoting agents with or without hyperthermia. For hyperthermic skin treatments, each group of mice was placed onto a platform in a water bath so that the dorsal skin of the mice was in contact with 44°C temperature controlled water.