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A method for hyperthermic treatment of mouse skin

Gragtmans, N. J., Jevcak, J. J., Mitchel, R. E. J., Morrison, D. P., McCann, R. A., Murphy, J. W.
Laboratory animals 1992 v.26 no.2 pp. 122-126
mice, skin, animal models, disease models, fever, carcinogenesis, carcinogens, promoter regions
The Sencar mouse skin system is a recognized model for tumour initiation, promotion and progression. The current interest in the effect of hyperthermia on this multi-stage tumorigenesis model prompted the need for a technique to accurately heat a section of dorsal skin of a large number of mice for 30 min per heat treatment. In the technique described, experimental groups of 25 female Sencar mice were treated at 7-8 weeks of age under general methoxyflurane anaesthesia. Treatment consisted of the application of initiating and/or promoting agents with or without hyperthermia. For hyperthermic skin treatments, each group of mice was placed onto a platform in a water bath so that the dorsal skin of the mice was in contact with 44°C temperature controlled water.