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Discrimination between the immature stages of Ceratitis capitata and Anastrepha fraterculus (Diptera: Tephritidae) populations by random amplified polymorphic DNA polymerase chain reaction
- Sonvico, A., Manso, F., Quesada-Allue, L.A.
- Journal of economic entomology 1996 v.89 no.5 pp. 1208-1212
- random amplified polymorphic DNA technique, pupae, Anastrepha fraterculus, larvae, population, Ceratitis capitata, genetic polymorphism, chemotaxonomy, Argentina
- A random amplified polymorphic DNA polymerase chain reaction method using 10-base long commercial random primers was used to identify polymorphisms in Argentine populations of 2 fruit fly pests. A fast and reliable discrimination between Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and Anastrepha fraterculus (Wiedemann) immature or mature stages was obtained using as few as 1 insect per assay The population of origin of immature individuals can be identified unambiguously using 2 primers.