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Esterase profiles of diazinon-susceptible and -resistant horn flies (Diptera: Muscidae)
- Guerrero, F.D., Pruett, J.H., Kunz, S.E., Kammlah, D.M.
- Journal of economic entomology 1999 v.92 no.2 pp. 286-292
- Haematobia irritans irritans, diazinon, toxicity, insecticide resistance, resistance mechanisms, esterases, acetylcholinesterase, enzyme activity, polyacrylamide gel electrophoresis, insect control
- A field population of horn flies, Hematobia irritans irritans (L.), exhibiting diazinon resistance in bioassays also displayed high overall esterase activity and organophosphate-insensitive acetylcholinesterase in dot blot activity assays. Native polyacrylamide gels yielded a complex qualitative and quantitative profile of esterases that varied considerably among individual flies from 9 populations, with 28 different esterase bands identified. The results from topical diazinon application experiments and subsequent analysis indicate that a number of specific esterases may contribute to diazinon resistance in the horn fly.