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A Pinus radiata AAA-ATPase, the expression of which increases with tree ageing
- Diego, L.B., Berdasco, M., Fraga, M.F., Canal, M.J., Rodriguez, R., Castresana, C.
- Journal of experimental botany 2004 v.55 no.402 pp. 1597-1599
- Pinus radiata, senescence, tree age, gene expression, adenosinetriphosphatase, buds, proteolysis, Arabidopsis thaliana, sequence homology, nucleotide sequences, complementary DNA, protein degradation, enzyme substrates, amino acid sequences
- Age-dependent changes in gene expression profiles were studied in vegetative Pinus radiata buds by means of differential display. Among several candidate cDNAs, a 327 bp fragment that shows high homology with an Arabidopsis thaliana 20S proteasome ATPase designated RPT5a was found. Northern hybridization confirmed that the accumulation of this transcript increases with tree ageing, suggesting a possible role of this AAA-ATPase gene in development-related specific proteolysis.