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In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds
- Abirached-Darmency, M., Abdel-gawwad, M. R., Conejero, G., Verdeil, J. L., Thompson, R.
- Journal of experimental botany 2005 v.56 no.418 pp. 2019-2028
- Medicago truncatula, Pisum sativum, peas, field crops, forage crops, gene expression, seed development, embryogenesis, embryo (plant), histology, cell differentiation, messenger RNA, nucleic acid hybridization, genes, storage proteins
- The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Transfer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff-naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.