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Description of the bacterium causing blight of leek as Pseudomonas syringae pv. porri (pv. nov.)

Samson, R., Shafik, H., Benjama, A., Gardan, L.
Phytopathology 1998 v.88 no.8 pp. 844-850
Pseudomonas syringae, new taxa, Allium porrum, plant diseases and disorders, taxonomy, pathogenicity, host range, immunology, defense mechanisms, blight, chemotaxonomy, numerical taxonomy, microbial physiology, nucleic acid hybridization, host plants, ice nucleation, hypersensitive response
Forty bacterial strains isolated from leek blight (Allium porrum) in France and other countries were studied by conventional biochemical methods, serological reactions, numerical taxonomy, DNA-DNA hybridization, and ice nucleation activity, as well as by pathogenicity on leek and other host plants. They were compared with reference strains of Pseudomonas, mainly pathotype strains of P. syringae pathovars and strains of P. syringae pv. syringae isolated from various host plants including onions. Leek strains sorted with P. syringae species (sensu lato) by LOPAT tests (production of levan-sucrase, oxidase, pectinase, arginine dihydrolase, and hypersensitive reaction on tobacco). Leek strains were pathogenic to leek and produced symptoms identical to those observed in the field. They were the only strains in our study that could cause blight of leek. Thus, our results justify the creation of a new pathovar. Leek strains constituted a highly homogeneous DNA group and a discrete phenon by numerical taxonomy, and they belonged to O-serogroup POR. The name of P. syringae pv. porri is proposed for the bacterium causing leek blight. Criteria for routine identification are presented and taxonomic status is discussed.