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VR-ACS6 is an auxin-inducible 1-aminocyclopropane-1-carboxylate synthase gene in mungbean (Vigna radiata)

Yoon, I.S., Mori, H., Kim, J.H., Kang, B.G., Imaseki, H.
Plant & cell physiology 1997 v.38 no.3 pp. 217-224
1-aminocyclopropane-1-carboxylic acid, Vigna radiata, seedlings, genetic techniques and protocols, complementary DNA, clones, genes, gene expression, benzyladenine, abscisic acid, hypocotyls, ethylene, biosynthesis, cycloheximide, dose response, enzyme activity, messenger RNA, nucleotide sequences, amino acid sequences, indole acetic acid, etiolation
We have isolated four cDNA clones of ACC synthase from etiolated mungbean seedlings treated with auxin. pVR-ACS2, pVR-ACS3 and pVR-ACS6 contained the same sequence as the previously reported DNA fragments, pMAC2, pMAC3 (Botella et al. 1992b) and pMBA1 (Kim et al. 1992), respectively. pVR-ACS1 was identical with pAIM-1 (Botella et al. 1992a). VR-ACS6 was specifically induced in response to the auxin signal. The IAA-induction of VR-ACS6 was very rapid (within 30 min) and insensitive to cycloheximide treatment at concentrations up to 100 micromolar. Significant accumulation of VR-ACS6 mRNA was detected at 1 micromolar IAA. The IAA-induced expression of VR-ACS6 was suppressed by ABA and ethylene, but enhanced by BA. These characteristics of VR-ACS6 expression were well correlated with the physiological data of auxin-induced ethylene production in mungbean hypocotyls. VR-ACS1 was strongly induced by cycloheximide, but was found to be not auxin-specific. Inhibitors of either ethylene biosynthesis (AOA) or action (NBD) increased the basal level of VR-ACS1 mRNA.