Membrane depolarization induced by N-acetylchitooligosaccharide elicitor in suspension-cultured rice cells
- Source:
- Plant & cell physiology 1997 v.38 no.8 pp. 902-909
- ISSN:
- 0032-0781
- Subject:
- Oryza sativa, cell suspension culture, cytoplasm, cell membranes, membrane potential, measurement, oligosaccharides, chitin, metabolites, defense mechanisms, phytoalexins, biosynthesis, chlorides, dose response, calcium, ionophores, ion transport, anaerobic conditions, azides, proton pump
- Abstract:
- N-acetylchitooligosaccharides (fragments of chitin) elicit defense responses, including phytoalexin production, in suspension-cultured rice cells. They induced rapid and transient membrane depolarization accompanied by a transient increase in net Cl- -efflux. The membrane depolarization was not affected by anaerobiosis or azide, suggesting that the major part of the depolarization was mediated by ion channels, not by energy-dependent ion pumps. Depolarization was partly inhibited in the presence of Ca2+- or Cl- -channel blockers and highly inhibited by depletion of Ca2+ in the extracellular medium. A calcium ionophore, A23187, caused a transient depolarization but not an increase in Cl- efflux, while it did not inhibit the elicitor-induced transient depolarization and Cl efflux. These suggest that the influx of Cn2+ from the extracellular space to the cytoplasm is necessary as an initial trigger but not sufficient for membrane depolarization, Cl- efflux, and the following signaling processes.
- Agid:
- 1402289
- https://doi.org/10.1093/oxfordjournals.pcp.a029250