U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.


Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.


Main content area

Membrane depolarization induced by N-acetylchitooligosaccharide elicitor in suspension-cultured rice cells

Kikuyama, M., Kuchitsu, K., Shibuya, N.
Plant & cell physiology 1997 v.38 no.8 pp. 902-909
Oryza sativa, cell suspension culture, cytoplasm, cell membranes, membrane potential, measurement, oligosaccharides, chitin, metabolites, defense mechanisms, phytoalexins, biosynthesis, chlorides, dose response, calcium, ionophores, ion transport, anaerobic conditions, azides, proton pump
N-acetylchitooligosaccharides (fragments of chitin) elicit defense responses, including phytoalexin production, in suspension-cultured rice cells. They induced rapid and transient membrane depolarization accompanied by a transient increase in net Cl- -efflux. The membrane depolarization was not affected by anaerobiosis or azide, suggesting that the major part of the depolarization was mediated by ion channels, not by energy-dependent ion pumps. Depolarization was partly inhibited in the presence of Ca2+- or Cl- -channel blockers and highly inhibited by depletion of Ca2+ in the extracellular medium. A calcium ionophore, A23187, caused a transient depolarization but not an increase in Cl- efflux, while it did not inhibit the elicitor-induced transient depolarization and Cl efflux. These suggest that the influx of Cn2+ from the extracellular space to the cytoplasm is necessary as an initial trigger but not sufficient for membrane depolarization, Cl- efflux, and the following signaling processes.