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Electrically induced protoplast fusion using pulse electric fields for dielectrophoresis
- Dimitrova, A.P., Christov, A.M.
- Plant physiology 1992 v.100 no.4 pp. 2008-2012
- Pisum sativum, protoplast fusion, mesophyll, protoplasts, calcium chloride, electric field, electrofusion
- The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formation of protoplast chains in the presence of CaCl2 up to 5 mM, and protoplast fusion in the presence of 3 mM CaCl2 were found when the pulse field was applied. The present results suggest the possibility of electrically induced protoplast fusion at cation concentrations that prevent fusion when sine-wave fields are applied.