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Cloning of a gene encoding raw-starch-digesting amylase from a Cytophaga sp. and its expression in Escherichia coli
- Jeang, C.L., Chen, L.S., Chen, M.Y., Shiau, R.J.
- Applied and environmental microbiology 2002 v.68 no.7 pp. 3651-3654
- Cytophaga, alpha-amylase, genes, nucleotide sequences, amino acid sequences, gene transfer, gene expression, enzyme activity, hydrolysis, corn starch
- A raw-starch-digesting amylase (RSDA) gene from a Cytophaga sp. was cloned and sequenced. The predicted protein product contained 519 amino acids and had high amino acid identity to alpha-amylases from three Bacillus species. Only one of the Bacillus alpha-amylases has raw-starch-digesting capability, however. The RSDA, expressed in Escherichia coli, had properties similar to those of the enzyme purified from the Cytophaga sp.