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Exopolysaccharide and kestose production by Lactobacillus sanfranciscensis LTH2590
- Korakli, M., Pavlovic, M., Ganzle, M.G., Vogel, R.F.
- Applied and environmental microbiology 2003 v.69 no.4 pp. 2073-2079
- Lactobacillus sanfranciscensis, sucrose, carbohydrate metabolism, polysaccharides, kestose, fermentation, fructooligosaccharides, fructose, sourdough, starter cultures
- The effect was investigated of sucrose concentration on sucrose metabolism and on the formation of exopolysaccharide (EPS) by Lactobacillus sanfranciscensis LTH2590 in pH-controlled fermentations with sucrose concentrations ranging from 20 to 160 g liter-1. The EPS production increased and the relative sucrose hydrolysis activity decreased by increasing the sucrose concentration in the medium. The carbon recovery decreased from 95% at a sucrose concentration of 30 g liter-1 to 58% at a sucrose concentration of 160 g liter-1 because of the production of an unknown metabolite by L. sanfranciscensis. This metabolite was characterized as a fructo-oligosaccharide. The oligosaccharide produced by L. sanfranciscensis was purified and characterized as a trisaccharide with a glucose/fructose ratio of 1:2. The comparison of the retention time of this oligosaccharide and that of pure oligosaccharide standards using two different chromatography methods revealed that the oligosaccharide produced by L. sanfranciscensis LTH2590 is 1-kestose. Kestose production increased concomitantly with the initial sucrose concentration in the medium.