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Bactericidal activity of glycinecin A, a bacteriocin derived from Xanthomonas campestris pv. glycines, on phytopathogenic Xanthomonas campestris pv. vesicatoria cells

Author:
Pham, H.T., Riu, K.Z., Jang, K.M., Cho, S.K., Cho, M.
Source:
Applied and environmental microbiology 2004 v.70 no.8 pp. 4486-4490
ISSN:
0099-2240
Subject:
Xanthomonas campestris pv. vesicatoria, Xanthomonas campestris pv. glycines, bacteriocins, antibacterial properties, biological control agents, plant pathogenic bacteria, cell membranes, membrane potential, membrane permeability
Abstract:
The ability of glycinecin A, a bacteriocin derived from Xanthomonas campestris pv. glycines 8ra, to kill closely related bacteria has been demonstrated previously by our group (S. G. Heu et al., Appl. Environ. Microbiol. 67:4105-4110, 2001). In the present study, we aimed at determining the glycinecin A-induced cause of death. Treatment with glycinecin A caused slow dissipation of membrane potential and rapid depletion of the pH gradient. Glycinecin A treatment also induced leakage of potassium ions from X. campestris pv. vesicatoria YK93-4 cells and killed sensitive bacterial cells in a dose-dependent manner. Sensitive cells were killed within 2 h of incubation, most likely due to the potassium ion efflux caused by glycinecin A. These results suggest that the bactericidal mechanism of action of glycinecin A is correlated with the permeability of membranes to hydroxyl and potassium ions, leading to the lethal activity of the bacteriocin on the target bacteria.
Agid:
1410416