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Direct production of ethanol from raw corn starch via fermentation by use of a novel surface-engineered yeast strain codisplaying glucoamylase and alpha-amylase
- Shigechi, H., Koh, J., Fujita, Y., Matsumoto, T., Bito, Y., Ueda, M., Satoh, E., Fukuda, H., Kondo, A.
- Applied and environmental microbiology 2004 v.70 no.8 pp. 5037-5040
- Saccharomyces cerevisiae, genetically engineered microorganisms, gene transfer, gene expression, Rhizopus oryzae, glucan 1,4-alpha-glucosidase, Streptococcus bovis, alpha-amylase, corn starch, alcoholic fermentation, ethanol production
- Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis alpha-amylase by using the C-terminal-half region of alpha-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.