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The adnA transcriptional factor affects persistence and spread of Pseudomonas fluorescens under natural field conditions

Author:
Marshall, B., Robleto, E.A., Wetzler, R., Kulle, P., Casaz, P., Levy, S.B.
Source:
Applied and environmental microbiology 2001 v.67 no.2 pp. 852-857
ISSN:
0099-2240
Subject:
Pseudomonas fluorescens, transcription factors, mutants, transposons, agricultural soils, colonizing ability, population density, mortality
Abstract:
A soil plot was inoculated with a mixture of Pseudomonas fluorescens PF0-2, the wild type, and Pf0-5a, a Tn5 insertion mutant in adnA, at 7.84 log CFU/g of soil. Over a period of 231 days, culturable populations of both strains were measured at selected times below and away from the point of inoculation. Pf0-5a did not spread as fast and attained significantly lower populations than Pf0-2. At sample depths below the inoculation site, the adnA mutant showed a significant decrease in CFU/g of soil as compared to Pf0-2. Pf0-2 was first detected at the 1.5-cm annular site at 3 days after inoculation, whereas Pf0-5a required 7 days to travel the same distance. At this distance, the wild-type strain could be detected at a 21.5- to 25-cm depth, whereas Pf0-5a could be detected only as deep as 15.5 to 18 cm. At 4.5 cm from the site of inoculation and in soil fractions corresponding to 13 to 18 cm, Pf0-2 was the only strain detected. These results suggest that the transcription factor AdnA provides a fitness advantage in P. fluorescens, allowing it to spread and survive in soil under field conditions.
Agid:
1411238