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Synthesis of a low-molecular-weight form of exopolysaccharide by Bradyrhizobium japonicum USDA 110

Author:
Louch, H.A., Miller, K.J.
Source:
Applied and environmental microbiology 2001 v.67 no.2 pp. 1011-1014
ISSN:
0099-2240
Subject:
Bradyrhizobium japonicum, carbohydrate metabolism, polysaccharides, chemical composition, mannose, glucose, galacturonic acid, galactose, chemical structure
Abstract:
A novel extracellular low-molecular-weight polysaccharide was detected as a contaminant within extracellular cyclic beta-1,6-beta-1,3-glucan preparations from Bradyrhizobium japonicum USDA 110 cultures. Compositional analysis, methylation analysis, and nuclear magnetic resonance analysis revealed that this low-molecular-weight polysaccharide was composed of the same pentasaccharide repeating unit previously described for the high-molecular-weight form of the exopolysaccharide (EPS) synthesized by B. japonicum strains. Mass spectrometry analysis indicated that the size of this low-molecular-weight form of EPS was consistent with a dimeric form of the pentasaccharide repeating unit.
Agid:
1411253