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Some pharmacokinetic parameters of the trypanocidal drug homidium bromide in Friesian and Boran steers using an enyzme-linked immunosorbent assay (ELISA)

Author:
Murilla, G.A., Holmes, P.H., Peregrine, A.S., Eisler, M.C., Ndung'u, J.M.
Source:
Journal of veterinary pharmacology and therapeutics 1999 v.22 no.5 pp. 295-300
ISSN:
0140-7783
Subject:
zebu, Boran, intramuscular injection, interspecific variation, intravenous injection, enzyme-linked immunosorbent assay, steers, pharmacokinetics
Abstract:
Pharmacokinetic studies on the trypanocidal drug homidium bromide using a competitive enzyme immunoassay (detection limit 0.1 ng/mL are reported for non-infected Friesian and Boran steers following treatment with homidium bromide at a dose of 1.0 mg/kg b.w. Following intravenous (i.v.) treatment of Friesian steers (n = 5), the mean serum drug concentrations were 31.9 +/- 2.1 and 3.9 +/- 0.4 ng/mL at 1 and 24 h, respectively. The decline in serum drug concentration was tri-exponential with half-lives of 0.064 +/- 0.037 h for t(1/2 alpha), 7.17 +/- 1.87 h for t(1/2 beta) and 106.3 +/- 6.6 h for t(1/2 gamma) for distribution and elimination phases 1 and 2, respectively. Drug was detectable in serum for 17 days following treatment. The mean residence time (MRT) was 63.4 +/- 7.5 h. Following intramuscular (i.m. treatment of Friesian steers (n = 5), the drug concentration at 1 h after treatment was 72.5 +/- 2.2 ng/mL. This declined to 9.8 +/- 1.8 ng/mL at 24 h. Low concentrations of between 0.1 and 0.3 ng/mL remained in circulation for up to 90 days post-treatment. Following intramuscular treatment of Boran steers (n = 5), the mean serum drug concentration at 1 h after treatment was 112.1 +/- 40.3 ng/mL. By 24 h after treatment, the concentration had fallen to 13.0 +/- 3.3 ng/mL. Thereafter, the serum drug concentration-versus-time profile and the pharmacokinetic parameters obtained following non-compartmental analysis were similar to those obtained following intramuscular treatment of Friesian steers.
Agid:
1411454