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Arabidopsis AtcwlNV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificities

Author:
Coninck, B. de, Le Roy, K., Francis, I., Clerens, S., Vergauwen, R., Halliday, A.M., Smith, S.M., Laere, A. van, Ende, W. van den
Source:
Plant, cell and environment 2005 v.28 no.4 pp. 432-443
ISSN:
0140-7791
Subject:
Arabidopsis thaliana, kestose, substrate specificity, complementary DNA, Komagataella pastoris, gene expression, beta-fructofuranosidase, nucleotide sequences, enzyme substrates, enzyme activity, plant proteins
Abstract:
The genome of Arabidopsis thaliana contains six putative cell-wall type invertase genes (AtcwINV1-6). Heterologous expression of AtcwINV1, 3 and 6 cDNAs in Pichia pastoris revealed that the enzymes encoded by AtcwINV3 and 6 did not show invertase activity. Instead, AtcwINV3 is a 6-FEH and AtcwINV6 is a fructan exohydrolase (FEH) that can degrade both inulin and levan-type fructans. For AtcwINV6 it is proposed to use the term (6&1) FEH. In contrast, AtcwINV1 is a typical invertase. FEH activity was also detected in crude extracts of different parts of Arabidopsis. To verify that the FEH activity of AtcwINV3 and 6 were not artefacts of the heterologous expression system, the protein corresponding to AtcwINV3 was isolated from whole Arabidopsis plants and indeed showed only 6-FEH activity and no invertase activity. Although no fructans can be detected in Arabidopsis plants, it is shown that kestoses (trimers) can be synthesized in crude leaf extracts. The putative physiological significance of FEH in so-called nonfructan plants is discussed.
Agid:
1411900