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Increased ethanol production during growth of electric-field stimulated Kluyveromyces marxianus IMB3 during growth on lactose-containing media at 45 degrees C

Author:
Simpson, J., Brady, D., Rollan, A., Barron, N., McHale, L., McHale, A.P.
Source:
Biotechnology letters 1995 v.17 no.7 pp. 757-760
ISSN:
0141-5492
Subject:
Kluyveromyces marxianus, ethanol production, yields, lactose, culture media, beta-galactosidase, enzyme activity, waste utilization, electric field, culture filtrates
Abstract:
Following treatment of the thermotolerant ethanol producing yeast strain Kluyveromyces marxianus IMB3 with single electric field pulses of 2.4kV/cm, ethanol production was found to increase from 25 to 35% of the theoretical maximum yield within 20 hours, during growth on lactose-containing media (4%[w/v]). Following treatment with electric fields and addition of 1 mM MnCl2 to lactose-containing media, ethanol production almost doubled within a 30 hour period. In addition, low levels of functional beta-galactosidase activity could be detected in the extracellular culture filtrates, presumably as a result of electropermeabilization.
Agid:
1412802