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Cloning and expression of a cDNA encoding a cytoplasmic L5 ribosomal protein from alfalfa (Medicago sativa L.)
- Asemota, O., Breda, C., Sallaud, C., El Turk, J., Kozak, I. de., Buffard, D., Esnault, R., Kondorosi, A.
- Plant molecular biology 1994 v.26 no.4 pp. 1201-1205
- Medicago sativa, complementary DNA, ribosomes, proteins, nucleotide sequences, amino acid sequences, gene expression, messenger RNA
- A cDNA encoding a putative cytoplasmic ribosomal protein LS from alfalfa (MsRL5), the first sequence from higher plants, has been characterized. The derived amino acid sequence of 181 residues contains the L5 signature, is 72.2% identical to yeast ribosomal L5 and shares high identity with other RL5 peptides from eukaryotic origin. The sequence does not contain any signal or transit peptide and therefore might be cytoplasmic. In all alfalfa organs examined MsRL5 transcripts were detected at approximately equal levels.