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Seed set in Cichorium intybus L. by pollination of flowers developed in vitro

Castano, C.I., Profit, M.P. de.
Plant cell, tissue, and organ culture 1996 v.46 no.3 pp. 211-218
fertilization (reproduction), Cichorium intybus, roots, in vitro culture, flowers, pollination, embryogenesis, seed set, seed development, culture media, photoperiod, dose response, light, viability, methodology, cultivars, genetic variation, explants
Formation of viable seeds of Cichorium intybus L. was achieved in an in vitro system. Flower formation, pollination, fertilization, embryogenesis and seed development occurred in vitro on chicory root explants on culture medium lacking plant growth regulators. After flower induction under a 24-h daylength treatment, the explants were transferred to a 16-h daylength at 40 microeinsteins m-2 s-1 irradiance for pollination and further seed development. Negative results were obtained when root explants were maintained continuously under a 24-h daylength during the whole culture period. Lower seed set was obtained when the cultures were at low irradiance. The need of a dark period and adequate level of irradiance are suggested as important factors to obtain viable seeds. The developed in vitro system can be used as a model to study the factors controlling the reproductive processes, and for the study of self-incompatibility in chicory.