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A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria
- Gonzalez, D.H., Bonnard, G., Grienenberger, J.M.
- Current genetics 1993 v.24 no.3 pp. 248-255
- Triticum aestivum, structural genes, plant proteins, ribosomes, mitochondrial DNA, complementary DNA, nucleotide sequences, amino acid sequences, gene expression, messenger RNA, RNA editing, restriction mapping, transcription (genetics), open reading frames
- Sequence analysis of a transcribed region of the wheat mitochondrial (mt) genome revealed two open reading frames (orfs) coding for proteins of 589 and 174 amino acids. Both genes are co-transcribed in a 2.6-kb RNA. The largest orf codes for a hydrophobic protein which bears similarity to a bacterial protein involved in the biogenesis of c-type cytochromes. Its corresponding RNA sequence is fully edited at 34 positions. The second orf encodes a protein homologous to the amino-terminal third of E. coli ribosomal protein S1, corresponding to the ribosome-binding domain of this protein. Its RNA sequence is edited at four positions, one of the edits creating a stop codon. The presence of both proteins in wheat mitochondria was demonstrated using specific antibodies raised against fusion proteins obtained in E. coli from the corresponding cDNAs.