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A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria

Gonzalez, D.H., Bonnard, G., Grienenberger, J.M.
Current genetics 1993 v.24 no.3 pp. 248-255
Triticum aestivum, structural genes, plant proteins, ribosomes, mitochondrial DNA, complementary DNA, nucleotide sequences, amino acid sequences, gene expression, messenger RNA, RNA editing, restriction mapping, transcription (genetics), open reading frames
Sequence analysis of a transcribed region of the wheat mitochondrial (mt) genome revealed two open reading frames (orfs) coding for proteins of 589 and 174 amino acids. Both genes are co-transcribed in a 2.6-kb RNA. The largest orf codes for a hydrophobic protein which bears similarity to a bacterial protein involved in the biogenesis of c-type cytochromes. Its corresponding RNA sequence is fully edited at 34 positions. The second orf encodes a protein homologous to the amino-terminal third of E. coli ribosomal protein S1, corresponding to the ribosome-binding domain of this protein. Its RNA sequence is edited at four positions, one of the edits creating a stop codon. The presence of both proteins in wheat mitochondria was demonstrated using specific antibodies raised against fusion proteins obtained in E. coli from the corresponding cDNAs.