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DNA insertion system for complex yeast shuttle vectors
- Daniel, J.
- Current genetics 1995 v.27 no.4 pp. 309-311
- Saccharomyces cerevisiae, Escherichia coli, plasmid vectors, homologous recombination, genetic transformation, electroporation
- A DNA insertion system, termed marked homologous recombination, was devised for the construction of complex yeast shuttle plasmids. This system, which is efficient, rapid and easy to use, should contribute to our understanding of gene-gene interactions in yeast cells.