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Nitrite uptake by Chlamydomonas reinhardtii cells immobilized in calcium alginate
- Vilchez, C., Vega, J.M.
- Applied microbiology and biotechnology 1994 v.41 no.1 pp. 137-141
- nitrites, water pollution, Chlamydomonas reinhardtii, uptake mechanisms, calcium, alginates
- When an initial cell loading of about 30-40 micrograms chlorophyll (Chl).g-1 gel and alginate suspension of 3% (w/v) were used for immobilization of Chlamydomonas reinhardtii, the resulting cell beads showed optimum nitrite uptake rate, at 30 degrees C and pH 7.5. of 14 micromoles NO2(-).mg-1 Chl.h-1, the photosynthetic and respiratory activities being about 120 micromoles O2 produced.mg-1 Chl.h-1, and 40 micromoles O2 consumed.mg-1 Chl.h-1, respectively. The nitrite uptake activity required CO2 in the culture and persisted after 8 days of cells immobilization, or in the presence of 0.2 mM ammonium in the medium. Our data indicate that alginate-entrapped C. reinhardtii cells may provide a stable and functional system for removing nitrogenous contaminants from waste-waters.